Device

Part:BBa_K4218016:Design

Designed by: Wuhui Jiang   Group: iGEM22_YiYe-China   (2022-10-03)


Double toehold switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to further improve the detection accuracy of CRC compared to last year, we combined the detection of methylated SDC2 with methylated TFPI2 this year. We constructed a toehold switch consisting of the plasmids of double-toehold pSB1C3 and double-trigger pCOLADuetTM-1, and then we have a functional verification for the plasmids in the BL21 cells.


Source

We combined BBa_K3577001 and BBa_K3822000 to make a new part named the double toehold switch.

References